ADAM Substrate
Fluorescent Substrate: MCA-LAQAPhe(homo)RSK(Dnp)-NH2
Catalog Number: PEPMCA002m001, 1mg, 80 USD
Use:
This fluorescent peptide substrate can be used to assess activity of enzymes in the ADAM and MMP family. It is the MCA/Dnp version of PEPDAB005. Typically, the peptide is dissolved in DMSO to make a stock solution of about 10mM concentration. When used for in vitro assays, the substrate is often used at about 10uM concentration. For use with ADAM10 and 17, the buffer should consist of 25mM Tris, pH 8, and 6 x 10-4 Brij detergent. If human ADAM8 or ADAM12 are used, add enough CaCl2 to the aforementioned buffer to achieve a concentration of 10mM. For use with the MMPs, the buffer should contain 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM CaCl2, 5 µM ZnSO4, and 0.01% Brij-35. Excitation and emission wavelengths are 325 and 393 nm respectively.
Molecular Weight:
1315.4 g/mol
Purity:
Greater than 92% as assessed by HPLC and Mass Spectrometry.
Solubility:
1 mg/ml in water with 10% Formic acid
Appearance:
Yellow lyophilized powder
Shipping:
The peptide powder is shipped at room temperature.
Storage:
Upon receiving, the peptide should be stored at -70 oC. Avoid repeated freeze-thaw cycles. If dissolved in liquid (such as DMSO), aliquot into separate tubes to minimize the number of freeze-thaw cycles.
Stability:
Light Sensitive. Samples are stable up to 6 months at -70oC.
Reference:
Fluorescent substrates for the proteinases ADAM17, ADAM10, ADAM8, and ADAM12 useful for high-throughput inhibitor screening. Moss, M.L. and Rasmussen, F.H. (2007) Analytical Biochemistry;366(2):144-8.